Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 4th International Congress on Epigenetics & Chromatin London, UK.

Day 1 :

Keynote Forum

Marvin H Caruthers

University of Colorado, USA

Keynote: DNA analogues for CRISPER/CAS fidelity and exon skipping
OMICS International Epigenetics 2018 International Conference Keynote Speaker Marvin H Caruthers photo
Biography:

Marvin H Caruthers is a distinguished Professor at the University of Colorado. He is a Guggenheim Fellow, completed PhD at Northwestern University and Post-doctoral Studies at MIT. His interests include nucleic acids chemistry and biochemistry. Approximately 35 years ago, the methodologies for chemically synthesizing DNA/RNA were developed in his laboratory and incorporated into instruments for synthesizing DNA/RNA as used by biochemists, biologists and molecular biologists. He is the recipient of several academic and research awards including The National Academy of Sciences Award in the Chemical Sciences, The Prelog Medal, The Economists Award in Biotechnology and The US National Medal of Science. He is an elected member of The US National Academy of Sciences, The American Academy of Arts & Sciences, The National Inventors Hall of Fame and a Corresponding Member of the German Academy of Science Gottingen. He is also a co-founder of Amgen and Applied Biosystems.

Abstract:

New thiomorpholino oligonucleotide analogues (TMO) containing morpholino and deoxyribonucleoside joined through thiophosphate-phosphor inter nucleotide linkages were chemically synthesized. These analogues have higher melting temperatures when compared to natural DNA/RNA and DNA/RNA duplexes. Moreover, the TMO/RNA duplexes exhibit the A-form structure and are RNase H1 active. Treatment of HeLa cells with fluorescently labeled TMO and TMO/DNA chimeras demonstrated that these analogues were efficiently taken up by cells and stimulates biological activity in a HeLa cell dual luciferase assay. Recently thiomorpholino oligonucleotides were found to be more active than any other tested analogue in exon skipping assays with a mouse model for Duchenne muscular dystrophy. Recently imidoamidate DNA was synthesized. Imidoamidate DNA forms duplexes with complementary DNA, is positively charged and can be transfected in the absence of lipid, and is RNase H1 active. In collaboration with Agilent Technologies, we have developed methods and instruments for the chemical synthesis on glass chips of DNA and RNA containing upto 300 nucleotides per segment. RNA containing phosphonoacetate nucleotides at the 3’/5’ ends have much higher fidelity than any other analogue in the CRISPER/CAS system

Keynote Forum

Alexey V Fedulov

Alpert School of Medicine of Brown University–Rhode Island Hospital, USA

Keynote: Maternal exposure to environmental particles leads to transgenerational epigenetic transmission of asthma risk
OMICS International Epigenetics 2018 International Conference Keynote Speaker Alexey V Fedulov  photo
Biography:

Alexey V Fedulov has his expertise in Epigenetic Engineering and Transgenerational Epigenetic Studies. His lab has built a model of targeted reactivation of epigenetically silenced genes by fusion complexes comprised of DNA demethylase enzymes and sequence-specific DNA binding domains. His laboratory studies are in immune and epigenetic mechanisms of lung disease including early life asthma origins. The ultimate goal of the studies is to find novel therapeutic approaches by modulating epigenetic
control of gene expression.

Abstract:

environment. Maternal asthma history and especially gestational environmental exposures increase the risk of asthma in humans
and animal models. Exposure of pregnant mice to diesel exhaust particles (DEP) or concentrated urban air particles (CAP) results in
an increase in asthma susceptibility in F1 pups in our model. Here, we sought to test the hypothesis that this transmission continues
trans-generationally and occurs via epigenetic mechanisms.
Methodology & Theoretical Orientation: After the pregnant dams received intranasal instillations of particle suspensions or
control, their F1, F2 and F3 offspring were tested for ovalbumin allergy predisposition. We treated a subset of mice at F1 with a
DNMT inhibitor to test if this would abrogate the transmission of the phenotype. Moreover, we have previously shown that dendritic
cells (DCs) in the F1 progeny are key to the phenotype because they convey the disease predisposition upon isogenic transplant and
harbor DNA methylation aberrations; we therefore analyzed the DCs’ methylome (eRRBS) in this transgenerational study in all
three generations.
Findings: We found that the elevated asthmatic susceptibility after maternal exposure to particles during pregnancy persists into
F2 and with more variability, into F3 generations: low dose ovalbumin increased levels of eosinophils, IL-5 and IL-13 in bronchoalveolar
lavage and histopathologic changes of allergic airway disease, not seen/minimal in controls. The DCs in these generations
continued to display DNA methylation changes in several thousand loci, the number was diminishing towards F3. Lineages treated
with DNMT inhibitor at F1 no longer showed asthma susceptibility at F2 or F3.
Conclusion & Significance: The data indicate that pregnancy airway exposure to particles triggers transgenerationally transmitted
asthma susceptibility and suggests a mechanistic role for epigenetic alterations in DCs in this process.

OMICS International Epigenetics 2018 International Conference Keynote Speaker Anne Clémence Veillard  photo
Biography:

Anne Clémence Veillard is working in Diagenode s.a. (Belgium), to develop innovative technologies to study DNA methylation and histone marks since 2014. She is
fascinated by how epigenetics makes the link between the history and faith of a cell and its gene expression. That is why she studied DNA methylation profile of several
types of pluripotent stem cells during her PhD, at the National Institute of Agronomical Research (INRA-France). She believes that good technical tools are necessary to
support exciting research projects.

Abstract:

Epigenetics is crucial for the regulation of gene expression and has broad relevance in biological processes like development, disease
and response to the environment. Epigenomics, the study of the epigenetic state of the genome, is therefore a fast growing field.
Diagenode, as a long time expert and leader in developing optimized tools to study epigenetic marks such as histone post translational
modifications and DNA methylation, continuously innovates further to study the epigenetics. Diagenode’s latest innovation,
ChIPmentation, integrates both chromatin immunoprecipitation (ChIP) and library preparation within the same protocol to ensure
successful next-generation sequencing experiments. Chromatin immuno precipitation coupled with high throughput sequencing
(ChIP-seq) has become the gold standard for whole genome mapping of protein DNA interactions. ChIPmentation, developed by
Diagenode in collaboration with the CeMM (Vienna-Austria) is based on tagmentation technology and is a robust ChIP-seq solution
that permits the integration of sequencing library preparation into the ChIP experiment. This approach significantly reduces the
number of steps in the process. In addition, ChIPmentation has been integrated into the work flow of Diagenode’s IP-Star automated
platform for greater ease of use, simplicity and reproducibility. ChIPmentation is a robust technology that will enable the generation
of data quickly and reliably for epigenomics research.